High pressure-sensitive gene expression in Lactobacillus sanfranciscensis

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High pressure-sensitive gene expression in Lactobacillus sanfranciscensis.

Lactobacillus sanfranciscensis is a Gram-positive lactic acid bacterium used in food biotechnology. It is necessary to investigate many aspects of a model organism to elucidate mechanisms of stress response, to facilitate preparation, application and performance in food fermentation, to understand mechanisms of inactivation, and to identify novel tools for high pressure biotechnology. To invest...

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Acid stress-mediated metabolic shift in Lactobacillus sanfranciscensis LSCE1.

Lactobacillus sanfranciscensis LSCE1 was selected as a target organism originating from recurrently refreshed sourdough to study the metabolic rerouting associated with the acid stress exposure during sourdough fermentation. In particular, the acid stress induced a metabolic shift toward overproduction of 3-methylbutanoic and 2-methylbutanoic acids accompanied by reduced sugar consumption and p...

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The acid-stress response in Lactobacillus sanfranciscensis CB1.

Lactobacillus sanfranciscensis CB1, an important sourdough lactic acid bacterium, can withstand low pH after initial exposure to sublethal acidic conditions. The sensitivity to low pH varied according to the type of acid used. Treatment of LB: sanfranciscensis CB1 with chloramphenicol during acid adaptation almost completely eliminated the protective effect, suggesting that induction of protein...

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Exopolysaccharide and kestose production by Lactobacillus sanfranciscensis LTH2590.

The effect was investigated of sucrose concentration on sucrose metabolism and on the formation of exopolysaccharide (EPS) by Lactobacillus sanfranciscensis LTH2590 in pH-controlled fermentations with sucrose concentrations ranging from 20 to 160 g liter(-1). The EPS production increased and the relative sucrose hydrolysis activity decreased by increasing the sucrose concentration in the medium...

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Purification and characterisation of mannitol dehydrogenase from Lactobacillus sanfranciscensis.

Mannitol dehydrogenase (MDH) was purified and characterised from Lactobacillus sanfranciscensis. Two peptide fragments of MDH were N-terminally sequenced for the first time in the genus Lactobacillus. The purified enzyme had an apparent molecular mass of 44 kDa and catalysed both the reduction of fructose to mannitol and the oxidation of mannitol to fructose. The K(m) value for the reduction re...

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ژورنال

عنوان ژورنال: Brazilian Journal of Medical and Biological Research

سال: 2005

ISSN: 0100-879X

DOI: 10.1590/s0100-879x2005000800013